Optimisation of HTT-HAP40 purification using heparin affinity chromatography

In order to do any biochemical studies of the huntingtin protein, I must first purify the protein in large amounts. The current protocol for protein purification is adapted from other published protocols and requires a long incubation of clarified cell lysate with FLAG resin which means my protein is sitting around with proteases and other Read More …

Manuscript finished! Design and characterisation of mutant and wild-type huntingtin proteins produced from a toolkit of scalable eukaryotic expression systems

Hi folks! This past month or so I have been busy getting a manuscript draft prepared. This article details the HTT constructs we have made and shared with the community, how they can be used to make pure huntingtin protein of different polyQ lengths and characterisation of the protein samples that we made. We think this Read More …

Full-length huntingtin protein aggregation and first attempts to co-purify HTT-HAP40 from insect cells

This past week, I have been completing experiments in two different threads: 1. I have used the different polyQ length huntingtin protein samples I made previously in an experiment which measures how the protein molecules aggregate (stick together) when heated. This technique is called differential static light scattering (DSLS). I saw no difference in the Read More …