Large scale purification of full-length huntingtin Q23, Q46 and Q78 from insect cells

Hello lab scribbles readers! I have been busy in the lab making lots of protein for some upcoming electron microscopy experiments – all materials and methods can be found on Zenodo. These proteins are the complete full-length human huntingtin protein with either 23, 46 or 78 glutamine amino acids in the polyQ repeat stretch representing Read More …

Test Expression of Huntingtin Domain Constructs in Insect Cell Culture

I am back from vacation and other distractions and now have some new and exciting data to share with everyone. Following on the previous limited proteolysis experiment where I chopped up the protein into proteolyticaly stable nuggets which allowed me to work out the potential domain boundaries of the huntingtin protein, I designed constructs which Read More …