Test Expression of Huntingtin Domain Constructs in Insect Cell Culture

I am back from vacation and other distractions and now have some new and exciting data to share with everyone. Following on the previous limited proteolysis experiment where I chopped up the protein into proteolyticaly stable nuggets which allowed me to work out the potential domain boundaries of the huntingtin protein, I designed constructs which Read More …

Limited proteolysis of full-length huntingtin with chymotrypsin

I have received data from SPARC, Sick Kids Hospital Toronto, for the limited digest experiment I previously wrote about. This experiment aims to work out where the flexible linker regions of the protein structure are, therefore predicting which regions of the protein sequence correspond to folded domains.All of the experimental data is uploaded onto Zenodo. Very Read More …

Huntingtin linker sequence determination by computational methods – correspondence with Alex Holehouse

I firmly believe that the more scientists discuss their data with experts both within their field and outside of it, the better! Without peer review, constructive criticism and comprehensive evaluation of the data we all generate, we can not understand its limitations nor its value to others working in the same field. In this vein, Read More …