Over the past few weeks, I have been working on how best to disperse the scientific data I have with as many people as possible in the most effective way, as well as getting this blog up and running. I have decided to deposit all of my methods and data, in real-time, on Zenodo. This great platform, operated by the CERN Data Centre, allows scientists to share data and findings as well as to preserve them online.
My first Zenodo post can be found here. Please feel free to get in touch and tell me what you think. The uploaded document details some preliminary experiments I have completed on the limited proteolysis of huntingtin, a project which I hope will yield some interesting structural data soon or at least shed some light on part of the domain organisation of this protein.
The point of doing these experiments is that understanding the structural organisation of the huntingtin protein should improve our knowledge of the function of the protein which in turn may inform better therapeutic strategies for HD treatment. That’s the idea anyway! More on structure-function relationships of proteins coming shortly.
I was lucky enough for this work to be provided with huntingtin protein sample from Stefan Kochanek and Bin Huang, both of Ulm University. Also funded by the CHDI, they published last year a new method of scalable huntingtin protein production. A big thanks to Stefan and Huang for their support in this project.
Looking forward to more experiments and more updates for everyone, watch this space.