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Month: June 2016

Purification of huntingtin domain constructs (2016/06/03)

Posted onJune 3, 2016June 9, 2016Leave a comment

Today’s post details work which follows on from the test expression experiments where we screened different sections of the huntingtin gene to see which successfully permitted soluble expression of protein in our insect cell expression system. The aim of these experiments is to try and see whether we can make parts of the huntingtin protein Read More …

CategoriesData, General

Recent Posts

  • Updates on all the huntingtin samples I have made and what has been keeping me busy in the lab
  • Purifications of HTT and HTT-HAP40 from insect (Sf9) and mammalian (EXPI293F) expression systems
  • Summer travels showcasing the huntingtin protein toolkit
  • Identifying new members of the huntingtin interactome with BioID and other updates
  • Successful generation of fragments of the HTT protein and improving the purification procedure for the HTT-HAP40 complex

Recent Comments

  • racheljaneharding on Identifying new members of the huntingtin interactome with BioID and other updates
  • Dee Farry on Identifying new members of the huntingtin interactome with BioID and other updates
  • J.B. Shaw on Searching far and wide for proteins that bind huntingtin
  • J.B. Shaw on SAXS of huntingtin – a different approach to assess the structure of this protein
  • J.B. Shaw on SAXS of huntingtin – a different approach to assess the structure of this protein

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  • About Lab Scribbles
  • Huntington’s Disease
  • Bio
  • Contact
  • Acknowledgements
  • About Lab Scribbles
  • Huntington’s Disease
  • Bio
  • Contact
  • Acknowledgements