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Home >2016 >September
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Month: September 2016

Purifying full-length huntingtin protein from baculovirus expression system for EM analysis

Posted onSeptember 21, 2016September 21, 2016Leave a comment

Things are moving fast in the lab which is exciting as it means the project is moving forward quickly too. However, it does mean that I now have lots of data to share in this post! Firstly, thanks to very generous sharing of reagents from Dr. Ihn Sik Seong, we are now able to make Read More …

CategoriesData, General

Recent Posts

  • Updates on all the huntingtin samples I have made and what has been keeping me busy in the lab
  • Purifications of HTT and HTT-HAP40 from insect (Sf9) and mammalian (EXPI293F) expression systems
  • Summer travels showcasing the huntingtin protein toolkit
  • Identifying new members of the huntingtin interactome with BioID and other updates
  • Successful generation of fragments of the HTT protein and improving the purification procedure for the HTT-HAP40 complex

Recent Comments

  • racheljaneharding on Identifying new members of the huntingtin interactome with BioID and other updates
  • Dee Farry on Identifying new members of the huntingtin interactome with BioID and other updates
  • J.B. Shaw on Searching far and wide for proteins that bind huntingtin
  • J.B. Shaw on SAXS of huntingtin – a different approach to assess the structure of this protein
  • J.B. Shaw on SAXS of huntingtin – a different approach to assess the structure of this protein

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  • About Lab Scribbles
  • Huntington’s Disease
  • Bio
  • Contact
  • Acknowledgements
  • About Lab Scribbles
  • Huntington’s Disease
  • Bio
  • Contact
  • Acknowledgements