Month: October 2016
Screening EM conditions for full-length huntingtin Q78
Now that I have managed to purify the full-length huntingtin protein with 3 different polyQ lengths (Q23, Q46 and Q78), I can begin the more exciting experiments where I can try to understand how the protein molecule is folded and what its structure is. One method of doing this is called electron microscopy (EM). This Read More …
Large scale purification of full-length huntingtin Q23, Q46 and Q78 from insect cells
Hello lab scribbles readers! I have been busy in the lab making lots of protein for some upcoming electron microscopy experiments – all materials and methods can be found on Zenodo. These proteins are the complete full-length human huntingtin protein with either 23, 46 or 78 glutamine amino acids in the polyQ repeat stretch representing Read More …