Cloning, expression and purification of brand new huntingtin constructs

Nearly all experiments to date have used full-length huntingtin cloned by Ji-Joon Song and Ihn Sir Seong, who kindly shared their expression constructs with us. Since then, we have cloned our own full-length expression constructs which can express full-length huntingtin protein in both insect cells AND mammalian cells. The wild-type (Q23) N-terminally tagged vector generated Read More …

Investigating huntingtin DNA binding

Studies which look for genome wide-associated risk factors (GWAS) have a shown a link between Huntington’s disease and DNA repair proteins as well as other DNA related proteins including splicing and transcription factors. Huntingtin was previously described as a DNA binding protein but I have not found studies using purified huntingtin protein and DNA samples in Read More …

CHDI Meeting Malta 2017

This week I am in Malta having a really exciting time meeting lots of fellow Huntington’s disease scientists at the CHDI Therapeutics Conference. We have had lots of interesting talks on huntingtin structure/function, huntingtin lowering strategies, and biomarkers. You can read about the talks on the HDBuzz twitter feed @HDBuzzfeed This afternoon we have been Read More …