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Month: March 2017

Purification of full-length huntingtin (HTT) constructs Q17 and Q46 from HEK293 cells

Posted onMarch 23, 2017March 23, 20176 Comments

Whilst the purification of the full-length huntingtin protein from the insect cell system we have working in the lab, gives high yields so plenty of protein to do experiments with, the protein is not made in a way which 100 % represents how it is made in humans. Human cells modify the protein with chemical Read More …

CategoriesData, General

Full-length huntingtin purification and cleavage of the N-terminal tag

Posted onMarch 22, 2017Leave a comment

Last week, I completed a purification of the full-length huntingtin protein Q23 from baculovirus expression system (BVES). This protein I hope to use in a series of binding assays to see if I can find any DNA or protein partners of the huntingtin protein. The current construct I have for protein expression (kindly provided by Read More …

CategoriesData, General

Purification of full-length huntingtin – trials and tribulations!

Posted onMarch 14, 2017December 12, 2018Leave a comment

I am back from vacation and time spent on other projects so now I am back in the lab working on my HD project. Unfortunately, it has not been smooth sailing, I have had numerous issues making the full-length huntingtin sample in the lab. The protein is consistently aggregating in the purification protocol but I Read More …

CategoriesData, General

Recent Posts

  • Updates on all the huntingtin samples I have made and what has been keeping me busy in the lab
  • Purifications of HTT and HTT-HAP40 from insect (Sf9) and mammalian (EXPI293F) expression systems
  • Summer travels showcasing the huntingtin protein toolkit
  • Identifying new members of the huntingtin interactome with BioID and other updates
  • Successful generation of fragments of the HTT protein and improving the purification procedure for the HTT-HAP40 complex

Recent Comments

  • racheljaneharding on Identifying new members of the huntingtin interactome with BioID and other updates
  • Dee Farry on Identifying new members of the huntingtin interactome with BioID and other updates
  • J.B. Shaw on Searching far and wide for proteins that bind huntingtin
  • J.B. Shaw on SAXS of huntingtin – a different approach to assess the structure of this protein
  • J.B. Shaw on SAXS of huntingtin – a different approach to assess the structure of this protein

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  • About Lab Scribbles
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  • About Lab Scribbles
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