This past 3 weeks I have been in the UK, working furiously to try to get a better handle on the EM structure solution pipeline, both in the lab and at the computer.
I was very fortunate to have the opportunity to prepare my huntingtin protein sample in Susan Lea’s lab, with lots of help from Justin Deme. This meant I could apply incredibly freshly prepared protein to the electron microscopy grids for structural analysis, instead of the freeze-thawed sample used previously, as I usually had to ship the sample on dry ice from Toronto to Oxford. It will be interesting to see if this makes a difference to the sample as it has been treated more gently, I certainly wouldn’t liked to be pulled in and out of the -80C freezer!
Here is the write up from the protein purification and gradient fixation steps of this process on Zenodo. More on this soon as I find time in between all of the cracking talks at the CHDI conference to get this completely written up.