Huntingtin has been reported to interact with the PRC2 complex. From the current literature, it is unclear if this is a direct physical interaction – Pubmed IDs: 26636336, 25574027, 23756188 and 19933700. As such, this experiment aimed to examine if an interaction of recombinant protein samples could be detected using a pull-down method.
Unfortunately the control reaction didn’t work so some tweaks need to be made to the protocol. Huntingtin seems to non-specifically bind the resin (also anecdotally reported to me) so I will likely have to immobilize the huntingtin first on the beads to avoid this hurdle…..