Purification of high mobility group box 1 protein (HMGB1) – a huntingtin interacting protein

To generate better data for cryo-EM analysis of the huntingtin protein structure, more stable and conformationally homogeneous samples may help improve the particle averages. Purifying complexes of huntingtin with proteins which specifically bind huntingtin may promote such sample stability. High mobility group box 1 (HMGB1)┬áis known to bind huntingtin but, to my knowledge, this interaction Read More …