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Month: August 2017

Experiments on huntingtin interacting protein HMGB1

Posted onAugust 14, 2017Leave a comment

To improve the samples I am making of huntingtin for structural studies, I am trying to find binding partners of the huntingtin protein. Complexes of huntingtin with binding partners may be more stable and amenable to study by structural methods. HMGB1 is one such reported huntingtin interacting protein. I have now tried to make this Read More …

CategoriesGeneral

Cryo-EM updates and more!

Posted onAugust 11, 2017August 11, 2017Leave a comment

This past few weeks have been busy with fellowship applications, writing grants and papers so I am rather behind on keeping you updated but there has been some exciting progress: Susan Lea has made fantastic progress processing cryo-EM data of our samples and we now have a 13 A envelope of the protein: https://zenodo.org/record/841824 2. On Read More …

CategoriesData, General

Recent Posts

  • Updates on all the huntingtin samples I have made and what has been keeping me busy in the lab
  • Purifications of HTT and HTT-HAP40 from insect (Sf9) and mammalian (EXPI293F) expression systems
  • Summer travels showcasing the huntingtin protein toolkit
  • Identifying new members of the huntingtin interactome with BioID and other updates
  • Successful generation of fragments of the HTT protein and improving the purification procedure for the HTT-HAP40 complex

Recent Comments

  • racheljaneharding on Identifying new members of the huntingtin interactome with BioID and other updates
  • Dee Farry on Identifying new members of the huntingtin interactome with BioID and other updates
  • J.B. Shaw on Searching far and wide for proteins that bind huntingtin
  • J.B. Shaw on SAXS of huntingtin – a different approach to assess the structure of this protein
  • J.B. Shaw on SAXS of huntingtin – a different approach to assess the structure of this protein

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  • About Lab Scribbles
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  • About Lab Scribbles
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