Experiments on huntingtin interacting protein HMGB1

To improve the samples I am making of huntingtin for structural studies, I am trying to find binding partners of the huntingtin protein. Complexes of huntingtin with binding partners may be more stable and amenable to study by structural methods.

HMGB1 is one such reported huntingtin interacting protein. I have now tried to make this protein 3 times (!) but so far have not been successful. There are some issues with the construct I am trying to use for the purification, so we will now spend some time recloning the construct: