Prof Ray Truant kindly shared his nanobody construct with us. This encodes a camelid derived Ig domain which binds huntingtin as he describes in this paper. This may be helpful in stabilizing our huntingtin protein samples to make it easier to work with in our structural experiments. Our cloning team recloned the nanobody DNA sequences Read More …

Hi folks! Recently, I have been doing some work to further characterise the huntingtin protein samples I am generating in the lab. My huntingtin protein is made in insect cells and these cells process proteins they make differently to human cells. In particular, I am keen to see whether a chemical modification called phosphorylation is Read More …