
Month: November 2017
Huntingtin nanobody expression and purification (2017/11/24)
Recently, I was kindly provided with nanobody constructs which binds the proline rich region of huntingtin exon 1 (the first 90 amino acids) by Ray Truant (McMaster University). Nanobodies are small antibody like molecules containing just a single Ig domain which have high affinity for a specific protein and ideally a specific part or motif Read More …
Mapping post-translational modifications of huntingtin from insect cells
I have been continuing to characterise the huntingtin protein samples I am generating in the lab. You can read about my first attempts to map post-translational modifications by mass spec here. Previously I found phosphorylation modifications on the huntingtin protein which are located on the same sites as huntingtin protein derived from human cells which Read More …