Twitter Googleplus Email LinkedIn
Menu
LabScribbles - Real-time open access science

LabScribbles

Real-time open access science

Primary Menu

Skip to content
  • About Lab Scribbles
  • Huntington’s Disease
  • Bio
  • Contact
  • Acknowledgements
Search
Home >2018 >January
Featured Image-1
Featured Image-2
Featured Image-3

Month: January 2018

SAXS of huntingtin – a different approach to assess the structure of this protein

Posted onJanuary 29, 2018January 29, 20183 Comments

We have generated some really interesting data from our cryo-EM studies of the huntingtin protein to date. Susan Lea and Justin Deme who are working on the HTT samples I send from Toronto, recently shared this screen capture of the huntingtin envelopes from the most recent sample which are super to see: As a complementary Read More …

CategoriesGeneral

More open notebookers and more open notebook updates!

Posted onJanuary 28, 2018Leave a comment

Its been a busy few weeks as we have been preparing and then launching the new exciting open notebook project from the SGC. 12 early career researchers are joining me to open up their lab notebooks on their various projects and we kicked off the launch with a super meeting in Ottawa, supported by the CIHR Read More …

CategoriesGeneral

Looking for huntingtin interacting partners – HMGB1 and iVHH4

Posted onJanuary 15, 20187 Comments

In my continued bid to find protein molecules which might bind and stabilize the huntingtin (HTT) protein molecule for my structural biology experiments, I have used a technique called pull-down to assess binding of the DNA binding protein HMGB1 as well as the HTT nanobody iVHH4 which I previously described and purified. In this case, Read More …

CategoriesGeneral

Huntingtin Intrabody and Nanobody Literature Review

Posted onJanuary 10, 20181 Comment

Happy new year! I hope that you all had a very happy holidays and that 2018 is treating you well so far. To kick off the new year, I have been doing some reading on huntingtin intrabodies and nanobodies and have written this up into an informal literature review. These are small Ig domain proteins Read More …

CategoriesData, General

Recent Posts

  • Updates on all the huntingtin samples I have made and what has been keeping me busy in the lab
  • Purifications of HTT and HTT-HAP40 from insect (Sf9) and mammalian (EXPI293F) expression systems
  • Summer travels showcasing the huntingtin protein toolkit
  • Identifying new members of the huntingtin interactome with BioID and other updates
  • Successful generation of fragments of the HTT protein and improving the purification procedure for the HTT-HAP40 complex

Recent Comments

  • racheljaneharding on Identifying new members of the huntingtin interactome with BioID and other updates
  • Dee Farry on Identifying new members of the huntingtin interactome with BioID and other updates
  • J.B. Shaw on Searching far and wide for proteins that bind huntingtin
  • J.B. Shaw on SAXS of huntingtin – a different approach to assess the structure of this protein
  • J.B. Shaw on SAXS of huntingtin – a different approach to assess the structure of this protein

Archives

  • December 2019
  • September 2019
  • May 2019
  • April 2019
  • March 2019
  • January 2019
  • December 2018
  • November 2018
  • October 2018
  • September 2018
  • August 2018
  • July 2018
  • May 2018
  • March 2018
  • January 2018
  • December 2017
  • November 2017
  • October 2017
  • September 2017
  • August 2017
  • July 2017
  • June 2017
  • May 2017
  • April 2017
  • March 2017
  • February 2017
  • January 2017
  • December 2016
  • November 2016
  • October 2016
  • September 2016
  • August 2016
  • July 2016
  • June 2016
  • May 2016
  • April 2016
  • March 2016
  • February 2016
  • January 2016

Categories

  • Communication
  • Data
  • General
  • Outreach

Meta

  • Log in
  • Entries feed
  • Comments feed
  • WordPress.org
  • [Un]Subscribe to Posts

Footer Menu

Skip to content
  • About Lab Scribbles
  • Huntington’s Disease
  • Bio
  • Contact
  • Acknowledgements
Menu
This website is licensed under a Creative Commons Attribution 4.0 International license.
  • About Lab Scribbles
  • Huntington’s Disease
  • Bio
  • Contact
  • Acknowledgements
  • About Lab Scribbles
  • Huntington’s Disease
  • Bio
  • Contact
  • Acknowledgements