
Month: January 2018
SAXS of huntingtin - a different approach to assess the structure of this protein
We have generated some really interesting data from our cryo-EM studies of the huntingtin protein to date. Susan Lea and Justin Deme who are working on the HTT samples I send from Toronto, recently shared this screen capture of the huntingtin envelopes from the most recent sample which are super to see: As a complementary Read More …
More open notebookers and more open notebook updates!
Its been a busy few weeks as we have been preparing and then launching the new exciting open notebook project from the SGC. 12 early career researchers are joining me to open up their lab notebooks on their various projects and we kicked off the launch with a super meeting in Ottawa, supported by the CIHR Read More …
Looking for huntingtin interacting partners - HMGB1 and iVHH4
In my continued bid to find protein molecules which might bind and stabilize the huntingtin (HTT) protein molecule for my structural biology experiments, I have used a technique called pull-down to assess binding of the DNA binding protein HMGB1 as well as the HTT nanobody iVHH4 which I previously described and purified. In this case, Read More …
Huntingtin Intrabody and Nanobody Literature Review
Happy new year! I hope that you all had a very happy holidays and that 2018 is treating you well so far. To kick off the new year, I have been doing some reading on huntingtin intrabodies and nanobodies and have written this up into an informal literature review. These are small Ig domain proteins Read More …