
Month: May 2018
Full-length huntingtin protein aggregation and first attempts to co-purify HTT-HAP40 from insect cells
This past week, I have been completing experiments in two different threads: 1. I have used the different polyQ length huntingtin protein samples I made previously in an experiment which measures how the protein molecules aggregate (stick together) when heated. This technique is called differential static light scattering (DSLS). I saw no difference in the Read More …
New folks in open notebooking!
Aside from work in the lab, I have been busy promoting the cause of open notebooking at a number of different events around the world. This has been very rewarding and I have enjoyed engaging different audiences in the work our open notebook community is doing. I spoke recently at the Creative Commons Global Summit Read More …
Full-length and exon 1 huntingtin protein purification from bacteria, insect and mammalian cells
Recently I have been busy making lots of huntingtin protein samples for various experiments I have planned shortly to work out what the protein is doing with respect to binding DNA and other nucleic acid material. I have been using constructs we made in house which have either N-terminal or C-terminal FLAG tags and a Read More …