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Month: July 2018

The ongoing quest for huntingtin interaction partners

Posted onJuly 19, 2018Leave a comment

This past week I have been replenishing my stocks of huntingtin samples making both the full-length Q42 protein as well as the full-length Q23 protein in complex with HAP40. The protocol for HTT-HAP40 complex generation is now very robust and the sample looks top notch. I have also tried to make HTT complexes with other Read More …

CategoriesGeneral

Starting new projects in huntingtin structural biology

Posted onJuly 10, 2018July 10, 2018Leave a comment

Hi folks! This last month or so has been a bit of a whirlwind. I was ecstatic to receive the HDSA career development fellowship and we were also successful in our funding applications to the Huntington Society of Canada as well as the CHDI Foundation. There will be some slight redirection in the projects but Read More …

CategoriesData, General

Recent Posts

  • Updates on all the huntingtin samples I have made and what has been keeping me busy in the lab
  • Purifications of HTT and HTT-HAP40 from insect (Sf9) and mammalian (EXPI293F) expression systems
  • Summer travels showcasing the huntingtin protein toolkit
  • Identifying new members of the huntingtin interactome with BioID and other updates
  • Successful generation of fragments of the HTT protein and improving the purification procedure for the HTT-HAP40 complex

Recent Comments

  • racheljaneharding on Identifying new members of the huntingtin interactome with BioID and other updates
  • Dee Farry on Identifying new members of the huntingtin interactome with BioID and other updates
  • J.B. Shaw on Searching far and wide for proteins that bind huntingtin
  • J.B. Shaw on SAXS of huntingtin – a different approach to assess the structure of this protein
  • J.B. Shaw on SAXS of huntingtin – a different approach to assess the structure of this protein

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  • About Lab Scribbles
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  • About Lab Scribbles
  • Huntington’s Disease
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