Previously I showed that I could reproduce the expression and purification of HTT-HAP40, shown by Guo et al in their Nature paper earlier this year. Now I have been able to make this same complex but with polyQ-expanded HTT. It would seem that HAP40 also stabilises the Q54 HTT molecule as much as the Q23 HTT molecule … Continue reading Expression and purification of the HTT-HAP40 complex with expanded polyQ (Q54)