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Month: October 2018

Biophysical investigation of HTT and HTT-HAP40 using DSLS and SAXS

Posted onOctober 14, 2018December 11, 2018Leave a comment

Hi folks! Over the past month or so, I have been conducting biophysical characterisation experiments of the HTT and HTT-HAP40 samples I am able to purify in the lab. I have used DSLS which measures thermal stability by monitoring protein aggregation over a temperature gradient. In an ongoing collaborative effort with Sasha Lemak and Lixin Read More …

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Recent Posts

  • Updates on all the huntingtin samples I have made and what has been keeping me busy in the lab
  • Purifications of HTT and HTT-HAP40 from insect (Sf9) and mammalian (EXPI293F) expression systems
  • Summer travels showcasing the huntingtin protein toolkit
  • Identifying new members of the huntingtin interactome with BioID and other updates
  • Successful generation of fragments of the HTT protein and improving the purification procedure for the HTT-HAP40 complex

Recent Comments

  • racheljaneharding on Identifying new members of the huntingtin interactome with BioID and other updates
  • Dee Farry on Identifying new members of the huntingtin interactome with BioID and other updates
  • J.B. Shaw on Searching far and wide for proteins that bind huntingtin
  • J.B. Shaw on SAXS of huntingtin – a different approach to assess the structure of this protein
  • J.B. Shaw on SAXS of huntingtin – a different approach to assess the structure of this protein

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  • About Lab Scribbles
  • Huntington’s Disease
  • Bio
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  • Acknowledgements
  • About Lab Scribbles
  • Huntington’s Disease
  • Bio
  • Contact
  • Acknowledgements