Optimisation of HTT-HAP40 purification using heparin affinity chromatography

In order to do any biochemical studies of the huntingtin protein, I must first purify the protein in large amounts. The current protocol for protein purification is adapted from other published protocols and requires a long incubation of clarified cell lysate with FLAG resin which means my protein is sitting around with proteases and other … Continue reading Optimisation of HTT-HAP40 purification using heparin affinity chromatography