Mapping post-translational modifications of huntingtin from insect cells

I have been continuing to characterise the huntingtin protein samples I am generating in the lab. You can read about my first attempts to map post-translational modifications by mass spec here. Previously I found phosphorylation modifications on the huntingtin protein which are located on the same sites as huntingtin protein derived from human cells which Read More …

Making huntingtin nanobodies

Prof Ray Truant kindly shared his nanobody construct with us. This encodes a camelid derived Ig domain which binds huntingtin as he describes in this paper. This may be helpful in stabilizing our huntingtin protein samples to make it easier to work with in our structural experiments. Our cloning team recloned the nanobody DNA sequences Read More …

Post-translational modifications of insect cell derived huntingtin and success with HMGB1

Hi folks! Recently, I have been doing some work to further characterise the huntingtin protein samples I am generating in the lab. My huntingtin protein is made in insect cells and these cells process proteins they make differently to human cells. In particular, I am keen to see whether a chemical modification called phosphorylation is Read More …