Making huntingtin domains and exciting EM updates

This past week I have been trying to pin down some of the problems I have been having with making huntingtin proteins. Whilst the full-length protein prep is optimised, making smaller regions of the protein has been somewhat¬†problematic with inconsistent yields between preps. This past week with the help of our excellent eukaryotic expression system Read More …

Happy New Year!

Happy new year! 2017 is well and truly underway and progress is ongoing for the huntingtin structure project. Prof Susan Lea has been busy collecting data for me on her microscope throughout the holidays. This approach, called cry-electron microscopy, which allows us to image individual particles of the huntingtin molecule. Susan has worked hard to Read More …

Large scale purification of full-length huntingtin Q23, Q46 and Q78 from insect cells

Hello lab scribbles readers! I have been busy in the lab making lots of protein for some upcoming electron microscopy experiments – all materials and methods can be found on Zenodo. These proteins are the complete full-length human huntingtin protein with either 23, 46 or 78 glutamine amino acids in the polyQ repeat stretch representing Read More …