Happy New Year!

Happy new year! 2017 is well and truly underway and progress is ongoing for the huntingtin structure project. Prof Susan Lea has been busy collecting data for me on her microscope throughout the holidays. This approach, called cry-electron microscopy, which allows us to image individual particles of the huntingtin molecule. Susan has worked hard to Read More …

Large scale purification of full-length huntingtin Q23, Q46 and Q78 from insect cells

Hello lab scribbles readers! I have been busy in the lab making lots of protein for some upcoming electron microscopy experiments – all materials and methods can be found on Zenodo. These proteins are the complete full-length human huntingtin protein with either 23, 46 or 78 glutamine amino acids in the polyQ repeat stretch representing Read More …

Test Expression of Huntingtin Domain Constructs in Insect Cell Culture

I am back from vacation and other distractions and now have some new and exciting data to share with everyone. Following on the previous limited proteolysis experiment where I chopped up the protein into proteolyticaly stable nuggets which allowed me to work out the potential domain boundaries of the huntingtin protein, I designed constructs which Read More …