Huntingtin linker sequence determination by computational methods – correspondence with Alex Holehouse

I firmly believe that the more scientists discuss their data with experts both within their field and outside of it, the better! Without peer review, constructive criticism and comprehensive evaluation of the data we all generate, we can not understand its limitations nor its value to others working in the same field. In this vein, Read More …

Domain mapping of full-length huntingtin by limited proteolysis with chymotrypsin – continued

Yesterday, I managed to overcome my eagerness to see huntingtin fragments on a gel and restained overnight with blue-silver coomassie to reveal fairly respectable bands, much more amenable to subsequent extraction from the gel and mass spectrometry analysis. All of the my write up for yesterday is on Zenodo. The team at SPARC, Sick Kids Read More …

Domain mapping of full-length huntingtin by limited proteolysis with chymotrypsin

I am continuing to try and understand the domain architecture of the huntingtin protein. In a bid to validate my previous limited proteolysis/mass spectrometry experiment, I wanted to see if I could get similar proteolytically stable fragments by digesting my remaining aliquot (courtesy of the generous Stefan Kochanek and Bin Huang) of full-length huntingtin protein Read More …