I have received data from SPARC, Sick Kids Hospital Toronto, for the limited digest experiment I previously wrote about. This experiment aims to work out where the flexible linker regions of the protein structure are, therefore predicting which regions of the protein sequence correspond to folded domains.All of the experimental data is uploaded onto Zenodo.
Very similar proteolytically stable regions of huntingtin were mapped. despite limited cleavage of the protein with a different enzyme. This evidence further supports the domain mapping described previously.
I am now eagerly awaiting the insect cell construct test expression data, to see if we can make some of these putative domains recombinantly.