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Month: July 2016

Follow up analysis from huntingtin test expression experiments

Posted onJuly 20, 2016July 20, 2016Leave a comment

Previously I reported some exciting test expression data where we showed that small chunks of the huntingtin protein could be made in the lab, a key milestone to aid future experiments where we try and work out what the huntingtin protein looks like and what it might be doing in the cells of our bodies. Since Read More …

CategoriesData, General

Rerun of the Test Expression of Huntingtin Domain Constructs (2016/07/06)

Posted onJuly 6, 2016Leave a comment

The last run of experiments where I have been trying to make huntingtin protein in insect cells have not been very successful. However, this has not deterred me! Looking back on the previous test expression of all of the different huntingtin fragments, there are a number of issues with the data so I decided to Read More …

CategoriesGeneral

Recent Posts

  • Updates on all the huntingtin samples I have made and what has been keeping me busy in the lab
  • Purifications of HTT and HTT-HAP40 from insect (Sf9) and mammalian (EXPI293F) expression systems
  • Summer travels showcasing the huntingtin protein toolkit
  • Identifying new members of the huntingtin interactome with BioID and other updates
  • Successful generation of fragments of the HTT protein and improving the purification procedure for the HTT-HAP40 complex

Recent Comments

  • racheljaneharding on Identifying new members of the huntingtin interactome with BioID and other updates
  • Dee Farry on Identifying new members of the huntingtin interactome with BioID and other updates
  • J.B. Shaw on Searching far and wide for proteins that bind huntingtin
  • J.B. Shaw on SAXS of huntingtin – a different approach to assess the structure of this protein
  • J.B. Shaw on SAXS of huntingtin – a different approach to assess the structure of this protein

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  • About Lab Scribbles
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  • About Lab Scribbles
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