
Month: March 2017
Purification of full-length huntingtin (HTT) constructs Q17 and Q46 from HEK293 cells
Whilst the purification of the full-length huntingtin protein from the insect cell system we have working in the lab, gives high yields so plenty of protein to do experiments with, the protein is not made in a way which 100 % represents how it is made in humans. Human cells modify the protein with chemical Read More …
Full-length huntingtin purification and cleavage of the N-terminal tag
Last week, I completed a purification of the full-length huntingtin protein Q23 from baculovirus expression system (BVES). This protein I hope to use in a series of binding assays to see if I can find any DNA or protein partners of the huntingtin protein. The current construct I have for protein expression (kindly provided by Read More …
Purification of full-length huntingtin – trials and tribulations!
I am back from vacation and time spent on other projects so now I am back in the lab working on my HD project. Unfortunately, it has not been smooth sailing, I have had numerous issues making the full-length huntingtin sample in the lab. The protein is consistently aggregating in the purification protocol but I Read More …