Last week, I was busy in the lab making different huntingtin protein samples for analysis by electron microscopy, experiments I will be doing with my collaborators Justin Deme and Susan Lea in Oxford this week hopefully.
I have made protein samples both with and without the N-terminal FLAG tag and have also made protein samples which have been oxidised – work in Ray Truants lab suggests this may affect the protein fold and function.
The samples arrived safe and sound on dry ice in Oxford this morning and I am looking forward to getting started by screening them by negative stain EM soon.
One thought on “Preparing huntingtin protein samples for analysis by electron microscopy”
Great post,Thanks for providing us this great knowledge