Month: May 2017
Investigating huntingtin DNA binding
Studies which look for genome wide-associated risk factors (GWAS) have a shown a link between Huntington’s disease and DNA repair proteins as well as other DNA related proteins including splicing and transcription factors. Huntingtin was previously described as a DNA binding protein but I have not found studies using purified huntingtin protein and DNA samples in Read More …
Purification and preparation of huntingtin samples for cryo-EM grid preparation in Oxford
This past 3 weeks I have been in the UK, working furiously to try to get a better handle on the EM structure solution pipeline, both in the lab and at the computer. I was very fortunate to have the opportunity to prepare my huntingtin protein sample in Susan Lea’s lab, with lots of help from Read More …