Cloning, expression and purification of brand new huntingtin constructs

Nearly all experiments to date have used full-length huntingtin cloned by Ji-Joon Song and Ihn Sir Seong, who kindly shared their expression constructs with us. Since then, we have cloned our own full-length expression constructs which can express full-length huntingtin protein in both insect cells AND mammalian cells. The wild-type (Q23) N-terminally tagged vector generated Read More …