Previously I showed that I could reproduce the expression and purification of HTT-HAP40, shown by Guo et al in their Nature paper earlier this year.

Now I have been able to make this same complex but with polyQ-expanded HTT. It would seem that HAP40 also stabilises the Q54 HTT molecule as much as the Q23 HTT molecule as I see a nice peak profile in the gel filtration step.

I hope to use this sample and the HTT-HAP40 Q23 samples to complete some biophysical studies.